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Image Search Results


MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and Ym1 in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.

Journal: Scientific Reports

Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

doi: 10.1038/s41598-025-97729-2

Figure Lengend Snippet: MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and Ym1 in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.

Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

Techniques: Activity Assay, Immunofluorescence, Staining, Fluorescence, Microscopy, Expressing, Western Blot, Control, Software

MLKL in myofibroblasts affects macrophages activities through PGE 2 . ( A ) The production of IL-6, NO and TNF-α in MLKL +/+ M1 macrophages after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. ( B ) The production of IL-10, arginase and Ym1 in MLKL +/+ M2ø after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

Journal: Scientific Reports

Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

doi: 10.1038/s41598-025-97729-2

Figure Lengend Snippet: MLKL in myofibroblasts affects macrophages activities through PGE 2 . ( A ) The production of IL-6, NO and TNF-α in MLKL +/+ M1 macrophages after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. ( B ) The production of IL-10, arginase and Ym1 in MLKL +/+ M2ø after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

Techniques: Control, Software

Antibodies used in Immunofluorescence staining.

Journal: Scientific Reports

Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

doi: 10.1038/s41598-025-97729-2

Figure Lengend Snippet: Antibodies used in Immunofluorescence staining.

Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

Techniques: Immunofluorescence, Staining, Concentration Assay

Antibodies used in Western blot.

Journal: Scientific Reports

Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

doi: 10.1038/s41598-025-97729-2

Figure Lengend Snippet: Antibodies used in Western blot.

Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

Techniques: Western Blot, Concentration Assay

Antibodies used in immunofluorescence staining

Journal: Cell Communication and Signaling : CCS

Article Title: NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice

doi: 10.1186/s12964-025-02063-9

Figure Lengend Snippet: Antibodies used in immunofluorescence staining

Article Snippet: Ym1 , Rabbit polyclonal , , 1:50 , Stemcell technologies , 60,130.

Techniques: Immunofluorescence

Antibodies used in Western blot

Journal: Cell Communication and Signaling : CCS

Article Title: NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice

doi: 10.1186/s12964-025-02063-9

Figure Lengend Snippet: Antibodies used in Western blot

Article Snippet: Ym1 , Rabbit polyclonal , , 1:50 , Stemcell technologies , 60,130.

Techniques: Western Blot, Concentration Assay